Application

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact [email protected].

56FHTEo- human tracheal epithelial cell line is a useful model for the study of epithelial ion transport, secretion and biochemistry.

Cell Line Description

Epithelial Cells

Disclaimer

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

56FHTEo- is a human fetal tracheal epithelial cell line that is immortalized with the origin-of-replication defective SV40 plasmid (pSVori-) . The cell line expresses the wild-type cystic fibrosis transmembrane conductance regulator (CFTR) and shows cAMP-dependent chloride ion transport and calcium ion-dependent chloride transport . The cell line is permissive for adenoviruses .

Quality

Each vial contains ≥ 1X10⁶ viable cells.
Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells are negative for mycoplasma contamination.
Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.